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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Estimation and Extraction of poly-ß- hydroxybutyrate in Bacillus cereus M13 with different conditions.

Author: NOORA AMIR, AL-WATIFIWEJDAN, RIDHA TAJ-AL-DEEN, ANWAR KADHM AL-SAFFAR
Abstract: quantity of synthetic plastic refuse incrementeach year addition to take long period to degradation, beside that produce toxic materials affecting in environment. This problem can solve by use bioplastic, the isolates from soil polluted by hydrocarbon materials in different position in Babylon/ Iraq. Bacillus cereus strainM13 was the isolate produce poly-ß-hydroxybutyrate in high yields. Tow fluorescent stain were used to screened about PHB production, Nile blue A and Nile red. the maximum PHB yields was appear when use glucose at 5% concentration as a carbon source, and yeast extract at concentration 0.5g/100ml as a nitrogen source. Regarding agitation speed, inoculum size, temperature, incubation time and pH, the optimum conditions of PHB yields were 150 rpm, 10%,37°C, 72 h and 8, respectively. Bacillus cereus strainM13 was capable of accumulating PHB from Glucose, Sucrose, Coffee waste , Rice bran, Wheat bran, Molasses and Potato waste that used as ananagrammatic carbon sources for the PHB yields. Production of PHB was elevated from 4.3% to 40% in HM medium.
Keyword: poly-ß-hydroxybutyrate, triosephosphateisomerase, optimization, Bacillus cereusM13.
DOI: https://doi.org/10.31838/ijpr/2019.11.02.028
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