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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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High Performance Thin Layer Chromatographic Method for Quantification of Gallic Acid in Root Powder of Alangium salvifolium (L.f) Wang

Author: UPLOADED BY-ADMIN, NATVARBHAI M PATEL, VINEET C JAIN, ANURAG BHARGAV, DHIREN P SHAH
Abstract: A sensitive, simple and accurate HPTLC method has been developed for the quantification of Gallic acid, a polyphenols in dry root powder of Alangium salvifolium (L.f) Wang. Chromatographic analysis was performed using chloroform, alcoholic and aqueous extracts, on silica gel 60F254 HPTLC plate, using a solvent system, comprising of Toluene: Ethyl acetate: Formic acid : Methanol, 6:6:1.6:0.4 (v/v/v/v) as mobile phase gives good separation of Gallic acid at Rf =0.40. Detection and quantification of Gallic acid was done by densitometric scanning at ? = 254 nm. The calibration curve of Gallic acid was found to be linear (200 -1200 ng/spot) dependent on the concentration against area. The equation of best fitting line was Y= 3639.1X + 130.86. The concentration of Gallic acid in the alcoholic extract and aqueous extract of dried root powder of Alangium salvifolium (L.f) Wang, by the proposed HPTLC method was found to be 0.07 and 0.04 µg. Gallic acid was found to be absent in chloroform extract. Correlation coefficient 0.9966 indicates good linearity between concentration and peak area. The identity of the Gallic acid band in the sample extracts was confirmed by overlaying the UV absorption spectrum of the sample with that from the reference standard Gallic acid, using the Camag TLC scanner 3. The proposed HPTLC method provided a good resolution of Gallic acid from other constituents present in the extracts can be used for a routine quality control analysis and quantification of Gallic acid in the root powder extracts of Alangium salvifolium (L.f) Wang.
Keyword: Alangium salvifolium (L.f) Wang, HPTLC, Gallic acid, Ankolam
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