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High Performance Thin Layer Chromatographic Method for Quantification of Gallic Acid in Root Powder of Alangium salvifolium (L.f) Wang
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| Author:
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UPLOADED BY-ADMIN, NATVARBHAI M PATEL, VINEET C JAIN, ANURAG BHARGAV, DHIREN P SHAH
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| Abstract:
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A sensitive, simple and accurate HPTLC method has been developed for the quantification of Gallic acid, a
polyphenols in dry root powder of Alangium salvifolium (L.f) Wang. Chromatographic analysis was performed
using chloroform, alcoholic and aqueous extracts, on silica gel 60F254 HPTLC plate, using a solvent system,
comprising of Toluene: Ethyl acetate: Formic acid : Methanol, 6:6:1.6:0.4 (v/v/v/v) as mobile phase gives good
separation of Gallic acid at Rf =0.40. Detection and quantification of Gallic acid was done by densitometric
scanning at ? = 254 nm. The calibration curve of Gallic acid was found to be linear (200 -1200 ng/spot) dependent
on the concentration against area. The equation of best fitting line was Y= 3639.1X + 130.86. The concentration
of Gallic acid in the alcoholic extract and aqueous extract of dried root powder of Alangium salvifolium
(L.f) Wang, by the proposed HPTLC method was found to be 0.07 and 0.04 µg. Gallic acid was found to be
absent in chloroform extract. Correlation coefficient 0.9966 indicates good linearity between concentration and
peak area. The identity of the Gallic acid band in the sample extracts was confirmed by overlaying the UV absorption
spectrum of the sample with that from the reference standard Gallic acid, using the Camag TLC scanner
3. The proposed HPTLC method provided a good resolution of Gallic acid from other constituents present in
the extracts can be used for a routine quality control analysis and quantification of Gallic acid in the root powder
extracts of Alangium salvifolium (L.f) Wang.
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| Keyword:
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Alangium salvifolium (L.f) Wang, HPTLC, Gallic acid, Ankolam
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| DOI:
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