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Computational approach for siRNA Based RNA Interference of ATP synthase subunit alpha coding gene of different strains of Mycobacterium tuberculosis
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| Author:
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NEEMA TUFCHI, KUMUD PANT, ASHISH THAPLIYAL
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| Abstract:
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Tuberculosis is a major health concern and around one third of the population is infected globally. The causative agent
Mycobacterium tuberculosis is becoming resistant towards the available drug gradually and worsening the cases to
multiple drug resistance and extensive drug resistance. Besides developing resistance towards the drugs, the
bacterium is also clever enough that by infecting the host it modifies the host protein at cellular as well as at tissue
level. So, there is an extensive need to develop a therapeutic that can fight with tuberculosis. RNA interference is well
known technique for the silencing of the substantial genes in numerous biological systems. In the current study we
have performed the computational approach for designing possible siRNA molecules. Various tools like sequence
similarity check, target alignment, secondary structure prediction and RNA interaction evaluation was used to design
siRNA for the target. The siRNA and chemically modified si-RNA (cm-siRNA) for gene silencing of various strains of
Mycobacterium tuberculosis were rationally designed and authenticated by all the rules required for synthesis of siRNA
through computational method. ?Gbinding of the target and its predicted siRNA was calculated to be -29.26 kcal/mol
and between target and cm-siRNA was -27.91 kcal/mol. The designed siRNA might lead to knockdown the pathogenic
activity of the bacterium Mycobacterium tuberculosis.
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| Keyword:
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Tuberculosis, Mycobacterium tuberculosis, RNA interference, siRNA, cm-siRNA
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2019.11.02.008
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| Download:
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