|
Distribution of pslA among Local Isolates of Biofilm- Producing Pseudomonas aeruginosa
|
|
| Author:
|
HASSAN AL-SHEIKHLY, LAITHN. MUSLEH, HARITH.J.F. ALMATHKHURY
|
| Abstract:
|
16S rRNA gene sequence examination is an effective instrument for characterization of new pathogens in clinical
specimens. Akey component of colonization, biofilm formation, and protection of the pragmatic human pathogen
Pseudomonasaeruginosais the biosynthesis of the exopolysaccharide Psl.Extracellular polysaccharides,biofilm, are
secreted by microorganisms into the neighboring environment and are significant for surface attachment and keeping
structural safety within biofilms.Biofilm production is an important technique for the survival of P. aeruginosa,and its
association with antimicrobial resistance represents a defy for patient therapeutics. The aim of the current research is
to assess the antibiotic resistance manner and distribution of the pslA gene among biofilm producingP. aeruginosa
isolates, which have beengained from some hospitals in Baghdad, Iraq. Twenty- five P. aeruginosa isolates were
obtained fromDepartment of Biology, College of Science, University of Baghdad. TheP. aeruginosa isolates were
recognized using standard bacteriological techniques. Drug susceptibility test was done by disk diffusion technique for
all the isolates against five antimicrobial agents.DNA was extracted from twenty-fiveP. aeruginosa isolates, which were
selected as being resistant to gentamicin using the polymerase chain reaction(PCR). A specific primer pair was used to
amplify 16S rRNA by a conventional PCR technique. Biofilm development was measured by microtiter plate test. The
results of 16S rRNA showed that all 25 selected isolates were resistant to gentamicin harbored this gene. Biofilm
formation was observed in 24/25(96%) of the P. aeruginosa isolates. The possibility of biofilm formation was
remarkablyrelatedtothe resistance to gentamicin. In addition, the pslA gene was existed in all biofilm and non-biofilm
producing the selected isolates with a frequency of 100% (n = 25).16S rRNA sequencing can be used to identify
genetically atypical P. aeruginosa isolates from different origins. Theresults of the currentresearch well clarified that the
P. aeruginosa biofilm-forming isolates were more resistant to the tested antibiotics. What is more, because of wide
spreading, it appears that the pslA gene is associated with biofilm formation.
|
| Keyword:
|
Pseudomonas aeruginosa, 16S rRNA, pslA, PCR
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2019.11.02.018
|
| Download:
|
Request For Article
|
|
|