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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Molecular Characterization Based on Genetic Diversity Analysis For Fertility Restorer Genes in Rice(Oryza Sativa, L.) Using Microsatellite Markers

Author: BALQEES AL-, NIDHAL ABDUL H. AL-BDAIRI, MOHAMMED A.ALANBARI
Abstract: Rice as one of the most important agricultural crops has a putative potential for ensuring food security, The present study was carried out with the objective to assess genetic diversity among 15 rice (Oryza sativa L.)genotypes representative offer tility restorer genes using two specific primers of SSR markers. Results showed that the range of molecular size (147.333 - 344.366) bp. A total of 11 alleles were amplified with a mean of 5 alleles perlocus. The Polymorphic information content PIC values ranged from 0.5198 to 0.8006 with a mean of 0.6602.The lowest value for main allele frequency was 0.2333 in primer RM171 while the highest value was 0.6000 for primer RM216.two primers (RM171 and RM216) gave adistinctive genetic fingerprinting of 4 genotypes understudy Analysis of results obtained from Neighbor-joining dendrogram revealed that, grouped all the 15 rice genotypes into two major groups: one small cluster A and a large cluster B. The major group A comprised 6 varieties, while the cluster B comprised 9. depending on their geographic origin, their ancestor. The results suggest microsatellite markers as a useful tool for the estimation of genetic diversity and cultivar differentiation and present invaluable genetic information for future breeding.
Keyword: Rice, polymorphism information contents (PIC), SSR marker,RM171.
DOI: https://doi.org/10.31838/ijpr/2019.11.02.015
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