|
Pathogenicity of mutantAspergillusfumigatusMAFU isolate in the lung of mice during infection period
|
|
| Author:
|
SUZAN RASHID, MAYSARASAMER KHALAF, HANAA N. ABDULLAH
|
| Abstract:
|
The selected mutantseAspergillusfumigatus MAFUin vitro exhibited significant differences among each other and with
their parents in daily radial growth rate. Regarding pathogenic activity,histopathological sections of the lungs of
BALB/c strains were prepared from each mice treated and from control to be used for comparisons among the
groups . Pieces from lung were fixed in 10% formalin for 1 day cut at 5 µm thickness, and20 mice were inoculated
intranasal with 5*106 conidia ofemutantA.fumigatus (MAFU) in 20 ?l of PBS-0.01% Tween 20. Five mice per group
were sacrificed after (7), (14) and (21) days after infection. Histopathologicaleexamination of lung after 7 days
revealed severe neutrophil and macrophage infiltrate in the alveolar space with increase thickness of intra alveolar
septa, and emphysema were present, hypertrophy of artery muscular layer andecongestion of blood vessel,
aggregation of neutrophil in the lumen.At 14 daysepost-infection, the lung showed granulamotus lesion in the wall
of bronchiole and severe and defuse aggregation of mononuclear cells and inflammatory cell in the lumen of the
bronchiole, defuse with septet hyphae also shown and emphysema were present. The tissue of lung at 21 day post
of infection expressed severe died and life neutrophil filled in the lumen also monocyte type 2 proliferation, also
supportive exudates in the lumen of the bronchiole and erosion of the epithelial of the bronchi and epithelial
layerof the bronchie.
|
| Keyword:
|
A.fumigatus, mutation, lung, UV, Pathogenicitye
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2019.11.02.011
|
| Download:
|
Request For Article
|
|
|