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In vivo tumour inhibitory effect of a crystal protein from Bacillus thuringiensis isolate of Peninsular India
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| Author:
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THEKKEKARA BABU, THOMAS CHUBICKA, SASIDHARAN SALINI, DEVAKI GIRIJA, ACHUTHAN C RAGHAVAMENON
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| Abstract:
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Parasporins, a group of non-insecticidal crystal proteins from Bacillus thuringiensis (Bt) is being emerging as a potential anticancer drug candidate due to its preferential cytotoxicity towards neoplastic cells. Even though, a number of parasporins with specific cytotoxicity are reported, the studies on the effect of these proteins on in vivo tumour models are scanty. Present study evaluated the anti-tumour efficacy of a crystal protein purified from a non-insecticidal Bacillus thuringiensis isolate (KAU 41) native to Western Ghats of India. The proteolytically activated crystal protein was dose dependently cytotoxic to Dalton’s Lymphoma Ascites (DLA) and Ehrlich Ascites Carcinoma (EAC) cells in vitro with an IC50 (Concentration required for 50% Inhibition) of 16 and 21 µg/ml concentrations respectively. No hemolysis was observed when the protein was incubated with human RBC. The intra-tumor administration of protein in the EAC induced ascites tumour bearing animals could effectively increase its life span to 38.5 and 58.6% in 25 and 50 µg protein treated group respectively. Again in animals with DLA induced solid tumour, the protein administration of the same concentration in situ leads to the reduction of tumour volume to 47.4 and 76.4% when compared to untreated control. These data indicates that the non haemolytic and non insecticidal crystal protein of Bt isolate, KAU 41 have significant anticancer potential.
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Bacillus thuringiensis, crystal protein, cytotoxicity, in vivo antitumor, DLA, EAC.
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