|
The Inhibitory Efficiency of Flavonoid Extract of Black Mustard Seeds (Brassica nigra) and cold aqueous extract of Euphorbia prostrata L. on growth of Staphylococcus aureusinvitro and invivo
|
|
| Author:
|
HANAN NOURI
|
| Abstract:
|
The aim of this study is to evaluate the antibacterial activity for flavonoids extract for Brassica nigra and cold equeous
extract for Euphorbia prostrata plants on growth of Staphylococcus aureus in vitro and in vivo. Six bacterial species were
isolated and identified from different conditional cases like enteric infection, diarrhea and skin abses. These are
Staphylococcus aureus (36.52%), Escherichia coli ,(44.34%), Bacillus cereus (4.34%), Klebsiellapneumoniae (6.08%),
Pseudomonas aeruginosa (15.65%) and Proteus mirabilis (2.60%). The results showed that flavonoid extracts from
Brassica nigra is highly active in inhibition than cold equeous extract from Euphorbia prostrata, the inhibitory effect
appear to be at a concentration of (25) mg/ml for the flavonoids extract and there is an increase in diameter of
inhibition zones with the increase of conc. While the cold equeous extract at (75) mg/ml conc seem to be active on
growth at Staphylococcus aureus only. MIC values were determine to be at (50,70) mg/ml for flavonoid extract on
Staphylococcus aureus and Escherichia coli respectively and (80,90) mg/ml for cold equeous extract on the same
bacteria.The value of LD50 for cold equeous extract was (1383) mg/kg and for flavonoids extract(2500) mg/kg in mice.
Also this study proved that the animals infected with Staphylococcus aureus when injected with cold equeous extract
of what showed no activity in vivo and died after (2) days of treatment while the animals injected with flavonoids
extract gave high activity for Staphylococcus aureusinvivo in (250,300) mg/kg, with clinical symptoms through
maintaining the normal values of blood and showed no clinical symptoms after injecting the animals with the
flavonoids extract and bacteria, while animal injected with bacteria alone produced changing in blood values and
developed clear clinical symptoms ended with the death of animals.
|
| Keyword:
|
Brassica nigra , Euphorbia prostrata L. , invitro , invivo ,Staphylococcus aureus.
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2019.11.01.046
|
| Download:
|
Request For Article
|
|
|