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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

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Impact Factor: 1.55

Total Publications: 317

Total Citation: 491

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Impact Factor: 1.46

Total Publications: 326

Total Citation: 477

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Chamomile Extract Down Regulate the Expression Level of Pro-inflammatory Cytokine in PBMCs In Vitro.

Author: FAIZAH ALMALKI
Abstract: Chamomile, one of the most popular curative plants that possess anticancer, anti-inflammatory and scavenging activities. However, the influence of chamomile as an immunostimulatory or immunoregulator remains, for the most part, unknown. In our research, we have attempted to ascertain the immunomodulatory effects of chamomile extracts (Chx) using different methods on isolated human peripheral blood mononuclear cells (PBMCs) and elucidated the mechanism by which chamomile extract treatment enhance the immune system. MTT assay was performed to set the proliferation percentage of PBMCs well as the viability percentage after Chx treatment. Also, the expression levels of cytokines, interlukin-6 (IL-6) and tumor necrosis factor (TNF-a) were investigated based on mRNA expression and protein synthesis via semi-quantitative PCR and ELISA method. The obtained data revealed that treatment of PBMCs with Chx at a dose of 5,10, 15, 20 and 25µg/ml for 0 - 48 hour enhanced cell viability and proliferation in a time and dose following pattern. Cell proliferation in response to phytohemagglutinin (PHA) stimulation was increased to reach 85% vs 70% and 87.5% vs 67% after 24 and 48 h. respectively and significantly enhanced the viability. Furthermore, Chx significantly depleted intracellular IL-6 and TNF levels by 36% and 25% below that of untreated cells at a dose of 15µg/ml which represent the most effective dose. The findings indicated that chamomile extract is a potent immunomodulative that enhanced PBMCs proliferation and viability and downregulate the pro-inflammatory cytokines and these modulating effects might promote the immune response.
Keyword: Chamomile, one of the most popular curative plants that possess anticancer, anti-inflammatory and scavenging activities. However, the influence of chamomile as an immunostimulatory or immunoregulator remains, for the most part, unknown. In our research, we have attempted to ascertain the immunomodulatory effects of chamomile extracts (Chx) using different methods on isolated human peripheral blood mononuclear cells (PBMCs) and elucidated the mechanism by which chamomile extract treatment enhance the immune system. MTT assay was performed to set the proliferation percentage of PBMCs well as the viability percentage after Chx treatment. Also, the expression levels of cytokines, interlukin-6 (IL-6) and tumor necrosis factor (TNF-a) were investigated based on mRNA expression and protein synthesis via semi-quantitative PCR and ELISA method. The obtained data revealed that treatment of PBMCs with Chx at a dose of 5,10, 15, 20 and 25µg/ml for 0 - 48 hour enhanced cell viability and proliferation in a time and dose following pattern. Cell proliferation in response to phytohemagglutinin (PHA) stimulation was increased to reach 85% vs 70% and 87.5% vs 67% after 24 and 48 h. respectively and significantly enhanced the viability. Furthermore, Chx significantly depleted intracellular IL-6 and TNF levels by 36% and 25% below that of untreated cells at a dose of 15µg/ml which represent the most effective dose. The findings indicated that chamomile extract is a potent immunomodulative that enhanced PBMCs proliferation and viability and downregulate the pro-inflammatory cytokines and these modulating effects might promote the immune response.
DOI: https://doi.org/10.31838/ijpr/2019.11.01.024
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Impact Factor for five years is 1.55 (2012 - 2016)

Year 2011 - 2015 Impact Factor - 1.46 Total Publications - 326 Total Citations - 477