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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence

IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Analysis of aleuritic acid in hard resin of seedlac using precolumn derivatization using 2, 4 Dibromoacetophenone and estimation of its content in different strains of Kerria lacca kerr.

Author: SHAILESH SINGH, , BARIJ NAYAN SINHA, KUNAL MUKHOPADHYAY
Abstract: Lac resin is a mixture of cross-linked polyesters of closely related straight chain or cyclic aliphatic polyhydroxy acids and sesquiterpenic acids. The major contribution to the polyester framework of resin comes from aleuritic acid, i.e., 9, 10, 16-trihydroxy-hexadecanoic acid. A novel derivatization reversed phase High Performance Liquid Chromatographic (RP-HPLC) method with UV detection was developed for qualitative and quantitative analysis of aleuritic acid. Derivatization was carried out using p- bromo- phenacyl bromide and crown ether to yield chromophoric ester and this process was optimized for maximum ester yield. The method for isolation of hard resin from sticklac obtained from insect Kerria lacca kerr has been optimized and aleuritic acid content in each sample of hard resin obtained from sticklac samples of different strains of the insect, was estimated.
Keyword: Hard Resin, Aleuritic Acid, High Performance Liquid Chromatography
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