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NEUROPROTECTIVE ACTIVITY OF SARGASSUM WIGHTII AGAINST NEUROTOXICITY INDUCED CELL DEATH ON AN IN VITRO HUMAN NEUROBLASTOMA MODEL
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| Author:
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R.RAJESWARI , , K.JEYAPRAKASH
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| Abstract:
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Parkinson’s and Alzheimer’s diseases are the result of the progressive dysfunction involving
the central nervous system. Although, these diseases such as PD and AD have been widely
studied over the past decades. Efficient treatments for these troubling diseases are not yet
determined and only some therapies only for the time being improvement in certain
symptoms. The objective of the current work was to investigate the probable neuro protective
activity of S.wightii after the induction of neurotoxin 1-methyl-4-phenlypyridinium (MPP + ) in
SH-SY5Y human neuroblastoma cells. Neuro protective activity of S.wightii extract were
evaluated for cell viability analysis, and IC 50 determination, and intracellular reactive oxygen
species, and acetylcholine esterase activity. The exposure of SH-SY5Y cells to MPP + along
with extract reduces the cell death up to 80 % for varying concentration in cell viability
analysis. In the ROS assay, there was an increase in the free radical scavenging activity with
the extract’s rising concentration. It indicates that the S.wightii extract reduces the oxidative
stress produced by the neurotoxin. The acetylcholine esterase inhibitory activity also
increased while compared with standard drug Donepezil. These observed results suggested
that the methanolic extract of S.wightii can be a resource for novel drug for
neurodegenerative diseases.
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| Keyword:
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Sargassum wightii, SH-SY5Y neuroblastoma cell lines, Neuroprotective activity, MPP
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| EOI:
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| DOI:
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