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Molecular screening of E.colibiofilm and anantibiofilm activity ofCapparisspinosa L extracts.
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| Author:
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HWAZENAMERABDALNABI J, J. ABIDHUDA JASIMM. ALTAMEME
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| Abstract:
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Escherichia coli is one of the main causative agents of diarrhea and other intestinal diseases which due to their
virulently and colonization ability playing the main role in the development of virulent cariogenic biofilms. Different
cell surface and structure molecules were a reason for the development and composition of the biofilm formation
ofEscherichiacoli. This study designedto identify the E coli strains isolated from diarrheal patients using specific and
virulent gene and use of Capparis spinosa extract in inhibition of bacterial biofilm formation ability Three hundred
eleven stool samples were collecting from children with diarrhea attended to Hilla Teaching and Al-Hashmiya
hospitals from October 2017 – April 2018. Diagnosis of E. coli isolates depends on standard laboratoryprotocols and
molecular detection method using specific primers based on16srRNA gene as a genetic marker for confirmed
isolationEscherichiacoliby PCR. Only 45 isolates were showed positive results for these genes. Investigation of isolates
activity for biofilm formation were achieved byTissue culture plate (TCP) assay. From50isolates of E. coliwere
collected from children with diarrhea only 45 of these isolates gives positive results for 16sRNA gene.E. coliisolates
were biofilm former, high, moderate biofilm formation mode was account for (70%) and (30%) respectively while
there are no isolates expresednon biofilm formation. Regardingthe effect of flower and leaf of C. spinosa on biofilm
formation, the result of this study demonstrates flower hotaqueousextract effectively reduces the biofilm formation
to 33% moderate ability, 77% weak and no strength. While leaf extract at different concentrations not prevents the
biofilm formation by E.coli. Molecular technique by using specific primers based on 16srRNA intergenic spacer gene as
a genetic marker give accurate identification for E.coli. Capparis spinosa, extracts have an effect onE. coli biofilm
formation.
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| Keyword:
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Diarrhea, E.coli, biofilm formation, Capparisspinose, Molecular technique.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.04.105
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| Download:
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