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Molecular-based identification of spermatogenesis quality improvement within two weeks induced by combination of intense exercise and cinnamaldehyde in Wistarmale rats fed high fat diet
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| Author:
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WAFAA ABDULLAH
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| Abstract:
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Exercise, low fat diet, or supplementations alone could show improvement in spermatogenesis quality afterlong
period of times that could reach up to months especially in males who have high access to a high fat diet (HFD), so
the current work was generated to induce this improvement in less amount of time, ~ 2 weeks, using intense exercise
and cinnamaldehyde in Wistar male rats (WMR) fed an HFD. To initiate the experiment, 40 WMRs(fed an HFD for 4
weeks before the exposure to the experimental variables (EEV) and continued after that) were randomly divided into
4 groups in which a group received intense exercise and cinnamaldehyde (IECd), a group exposed to cinnamaldehyde
alone (Cd), another group subjected to intense exercise alone (IE), and a group given the carrier of CD (CrCd). The
EEV was lasted for 2 weeks, and the animals were subjected to measuring serum testosterone (ST), sperm counting
(SpC) of motile and viable sperms, and inducible nitric oxide synthase (iNOS) gene expression (iNOSge) using real
time-polymerase chain reaction (RT-PCR).The levels of the ST and SpC of motile and viable sperms were significantly
(p<0.05) increased in the IECd group when compared with those in the Cd, IE, and CrCd groups. No differences
(p>0.05) were noticed between Cd, IE, and CrCd groups when tested for the levels of ST and SpC. The iNOSge
levels were significantly (p<0.05) decreased in the IECd group when compared with those in the Cd, IE, and CrCd
groups. No differences (p>0.05) were noticed between Cd, IE, and CrCd groups when tested for the levels of
iNOSge. This study indicates important effects of the intense exercise and cinnamaldehyde when applied together in
improving the quality of spermatogenesis in Wistar rats that could be tested in the future on the human model.
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| Keyword:
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Cinnamaldehyde, high fat diet, intense exercise, spermatogenesis.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.04.101
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| Download:
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