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Molecular Identification of Specific Biofilm Genes in Multidrug-ResistantEscherichia coli Isolates
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| Author:
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HASSAN FADHILNAJI, THEKRA ABDUL-AALI AL-KA’ABI
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| Abstract:
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One hundred and sixty samples of therapeutic medical devices were collected from AL-Hilla hospitals in Babylon city
(Iraq) during the period from November 2017 to March 2018. Twenty-twoMultidrug-ResistantEscherichia coli isolates
were obtained, these isolates were molecularly identified using polymerase chain reaction (PCR) technique in addition
to morphological properties diagnosed using MaCconkey agar and eosin methylene blue agar (EMB). The antibiotics
susceptibility test revealed that the percentages as follows: ciprofloxacin (100%), ceftazidime (100%), metronidazole
(95%), cefotaxime (81%), erythromycin (77%), aztreonam (72%), cefixime (72.7%), nitrofurantoin (68%), tetracycline
(63%), meropenem (63.6%), norfloxacin (54.5%), imipenem (54.5%), amikacin (50%).polymerase chain reaction (PCR)
analysis showed the presence ofbiofilm genes rcsA (68.18%), rpos (100%), sdiA1 (45.5%) all isolates. The phylogenetic
groups genes (chuA, yjaA, and TspE4.C2) werefound in all isolates at the percentages of 77.72%, 80.72%and 70.72%,
respectively. These results suggested that the expression of antibioticresistant genes were linked with the presence of
biofilm genes in E. coliisolates.
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| Keyword:
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nitrofurantoin,cefixime
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.04.087
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| Download:
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