IJPR  articles are Indexed in SCOPUSClick Here     Impact Factor for Five Years is 0.13 (2013 - 2018).    

logo

INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence

IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
Current Issue
Article In Press
No Data found.
ADOBE READER

(Require Adobe Acrobat Reader to open, If you don't have Adobe Acrobat Reader)

Index Page 1
Click here to Download
IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

Click to download
 

Article Detail

Label
Label
Development and validation of stability indicating HPTLC method for determination of Rutin and Quercitin

Author: MRINALINI DAMLE, JYOTI VYAVAHARE
Abstract: A new simple and selective stability- indicating high performance thin layer chromatographic (HPTLC) method has been developed and validated for simultaneous estimation of Rutin and Quercitin. The chromatographic development was carried out on TLC plates Precoated with silica gel 60 F254 using mixture of Ethyl acetate: Toluene: Methanol: Formic acid (5:3:2:0.5) v/v as mobile phase. Both the markers showed considerable absorbance at 255 nm. Hence it was chosen as detection wavelength. Stress testing of Rutin and Quercitin was carried out according to the international conference of harmonization (ICH) guideline Q1A (R2). The markers were subjected to acid, base, neutral hydrolysis, oxidation, thermal degradation and photolysis. . The system showed a peak for Rutin and Quercitin at Rf value of 0.24 ± 0.02 and 0.68 ± 0.03, respectively. The method was successfully validated according to ICH guidelines Q2R1. The data of linear regression analysis indicated a good linear relationship over the range of 100–500 ng/band concentrations with correlation coefficient value greater than 0.995. The accuracy of the method was established based on the recovery studies. Under various stressed conditions, Rutin and Quercitin do not showed any degradation product under alkali, acidic, oxidative and neutral hydrolytic condition.
Keyword: Rutin, Quercitin, HPTLC, Stability-Indicating Method
Download: Request For Article
 












ONLINE SUBMISSION
USER LOGIN


Username
Password
Login | Register
News & Events
SCImago Journal & Country Rank

Terms and Conditions
Disclaimer
Refund Policy
Instrucations for Subscribers
Privacy Policy

Copyrights Form

0.12
2018CiteScore
 
8th percentile
Powered by  Scopus
Google Scholar

hit counters free