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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence

IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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In Vitro Cytotoxicity Screening of Coumarin Compounds on Hep2 Cancer Cell line

Author: UPLOADED BY-ADMIN, RAJESH M.PATEL, N.J.PATEL
Abstract: Development of new therapeutic drugs needs assay systems to evaluate the toxicity potential of the drugs early in the drug discovery process. In vitro cell-based cytotoxicity assay is an easy and costeffective tool for hit ranking and lead optimization at the early stage of drug discovery. In present study, short term cytotoxicity protocol was used to evaluate the acute toxicity potential of some coumarin derivatives on cancer cells. we have used the coumarin derivatives namely 4-hydroxycoumarin (Compound I), 5-chloro 4-hydroxycoumarin (Compound II) and 7-hydroxy 4-methylcoumarin(Compound III) for screening anti cancer potential on Hep2 cells (Human laryngeal carcinoma cells; cancer cells) and Vero cells (African green monkey kidney cells; Normal cells) by SRB, MTT and LDH assay and IC50 was determined which was then compared for all three assay in search of the effective method of cytotoxicity determination. The IC50 was determined by dose response curve analysis and statistical analysis, by plotting the graph of concentration vs % growth Inhibition. The IC50 value of Compound II and III were comparably lower than compound I on Hep2 cells in all three cytotoxicity methods proved that II & III are good candidate may possess excellent anti cancer potentials against various malignancies.
Keyword: IC50, Cytotoxicity, Coumarin derivatives.
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