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ANTI-BIOFILM ACTIVITY OF TERMINALIA CHEBULA FRUITS AGAINST ORAL PATHOGENS
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| Author:
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S.AISHA SITHIKA,, A.RENUKA, D.SHANMUGASUNDAR, ANEESH NAIR, FLORIDA TILTON
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| Abstract:
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Dental caries is a common oral bacterial pathology caused by a biofilm consisting of microorganisms present on the tooth
surface.Biofilm is an association of micro-organisms in which microbial cells adhere to each other on a living or non-living
surface within a self-produced matrix of extracellular polymeric substance. During biofilm formation many species of
bacteria are able to communicate with one another through specific mechanism called quorum sensing.Bacterial biofilm is
less accessible to antibiotics and human immune system and thus poses a big threat to public health because of its
involvement in variety of infectious diseases.This paper is designated to evaluate the antibacterial activity of three solvent
extracts of Terminalia chebula fruits against oral pathogens. The biofilm formation assay was carried out by Crystal violet
assay.The antibacterial activities were assessed by agar well diffusion.Antibiofilm activity was investigated by Crystal Violet
assay.Ethyl acetate and Ethanol extracts of Terminalia chebula showed the best antibacterial activities against both the
strains in the minimal inhibitory concentration ranged from 20-100 µg/mL .Killing ability depended on time and
concentrations of the extract. Terminalia chebula acts as a potent antibiofilm agent with dual actions, preventing and
eradicating the biofilm. The major constituent of Terminalia chebula extract was ethanol, which responded for antibacterial
and antibiofilm against oral pathogens. It suggests that the ethanol Terminalia chebula extract may be used for preventing
dental plaques.
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| Keyword:
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Biofilms,T.chebula, oral pathogens, Crystal Violet Assay, phytochemical analysis
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| EOI:
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http://eoi.citefactor.org/10.11209/ijpr.10.03.009
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.03.036
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| Download:
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