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APPLICATION OF CONDENSATION TECHNIQUE FOR THE ESTIMATION OF DARUNAVIR AND EFAVIRENZ IN PURE AND TABLET FORMULATIONS
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| Author:
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R.VIJAYALAKSHMI* , D. ANJANI, M.D. DHANARAJU
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| Abstract:
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This proposed work describes two simple and fast visible spectrophotometric methods for the estimation of Darunavir
with O-nitro benzaldehyde and Efavirenz with vanillin by the condensation process.Both the methods were developed
on Perkin Elmer LAMBDA 25 UV –VIS spectrophotometer interfaced with UV Win lab software and 1cm quartz cells.
The methods are based mainly on the reaction of the free amino group in the Darunavir with the ONB reagent and
Efavirenz with the vanillin reagent undergoing condensation reaction to form colored Schiff’s bases. The methods were
optimized as per standard optimization parameters.By the optimized method the pinkish brown colored chromogen for
condensed DARUNAVIR was measured at 486 nm and reddish pink colored chromogen for condensed EFZ was
measured at 432 nm against the reagent blank. The linearity range of Darunavir is 40-240 µg/ml and Efavirenz is 20-
100µg/ml; LOD and LOQ was found to be 2.66 and 8.87 µg/ml for Darunavir and 1.44 and 4.82 µg/ml for Efavirenz. The
colorimetric methods were extensively validated as per ICH guidelines and all the parameters were within the
acceptance criteria with the correlation of 0.9999 and % RSD less than 2 for both the methods. The results of the
accuracy studies were nearer to 100%. The methods were proved to be more accurate, simple, precise and rapid by
statistical validation as well as recovery studies and could be used for routine laboratory analysis.
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| Keyword:
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Darunavir (DNV), Efavirenz (EFZ), O-nitro Benzaldehyde (ONB), Vanillin
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2018.10.03.004
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| Download:
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