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Molecular detection of Toxoplasmagondiiand Cryptosporidium spp. Of some snakes in ALDiwaniyah province/Iraq
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| Author:
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SADIYA ANAHHADIM.HAMAZAAL-MAYALI
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| Abstract:
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The Molecular Study, used for the first time in Iraq to detect zoonosis parasites between snakes and humans, included
two species of parasites: Toxoplasma gondii and Cryptosporidium spp., the conventional polymerase chain reaction
technique was used to investigate the gene 18S rRNA(382bp)for Toxoplasma gondii in the snake tissue of the liver, heart,
kidney and brain , 5 samples gave positive result With a total infection rate %16.66 included 3(%60) samples of the
E.jaculus and one sample(%20) of P.ventromaculatus and one sample(%20) of M.monspessulana respectively, and the
highest percentage of the presence of the gene was recorded in liver of snakes(%80), and the percentage of the lowest
in brain %20 The presence of the gene was not recorded in both the heart and the kidney.Cryptosporidium parasite
where the conventional polymerase chain reaction technique was used as a first step to investigate the SSU
rRNA(298bp) gene for the differentiation species of parasites and the DNA sequencer technique, The same gene was
used to analyze the evolutionary tree of local isolators that represented C.parvum and (MEGA 6) to determine the ratios
of convergence between local isolators and International isolators recorded at the International Gene Bank (NCBI). The
results of the analysis of the genetic tree of local isolators showed three common ancestors of the genetic groups with
the International isolators registered at the International Gene Bank,The samples recorded in NCBI under accession
number MG878886, MG878885, MG878884, MG878437, MG878399, MG888048& MG888047.
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| Keyword:
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Snake , Reptile, Toxoplasma gondii,Cryptosporidium spp., Iraq
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| EOI:
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| DOI:
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