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Published by : Advanced Scientific Research
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Molecular detection of Toxoplasmagondiiand Cryptosporidium spp. Of some snakes in ALDiwaniyah province/Iraq

Abstract: The Molecular Study, used for the first time in Iraq to detect zoonosis parasites between snakes and humans, included two species of parasites: Toxoplasma gondii and Cryptosporidium spp., the conventional polymerase chain reaction technique was used to investigate the gene 18S rRNA(382bp)for Toxoplasma gondii in the snake tissue of the liver, heart, kidney and brain , 5 samples gave positive result With a total infection rate %16.66 included 3(%60) samples of the E.jaculus and one sample(%20) of P.ventromaculatus and one sample(%20) of M.monspessulana respectively, and the highest percentage of the presence of the gene was recorded in liver of snakes(%80), and the percentage of the lowest in brain %20 The presence of the gene was not recorded in both the heart and the kidney.Cryptosporidium parasite where the conventional polymerase chain reaction technique was used as a first step to investigate the SSU rRNA(298bp) gene for the differentiation species of parasites and the DNA sequencer technique, The same gene was used to analyze the evolutionary tree of local isolators that represented C.parvum and (MEGA 6) to determine the ratios of convergence between local isolators and International isolators recorded at the International Gene Bank (NCBI). The results of the analysis of the genetic tree of local isolators showed three common ancestors of the genetic groups with the International isolators registered at the International Gene Bank,The samples recorded in NCBI under accession number MG878886, MG878885, MG878884, MG878437, MG878399, MG888048& MG888047.
Keyword: Snake , Reptile, Toxoplasma gondii,Cryptosporidium spp., Iraq
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