|
ANTI-PROLIFERATIVE AND PRO-APOPTOTIC ACTIVITY OF CARVACROL ON HUMAN CANCER CELLS
|
|
| Author:
|
ATHEER YASEEN
|
| Abstract:
|
This study tested the anti-proliferative and pro-apoptotic activity of carvacrol;a compound commonly present in the
essential oil of oregano and thyme.The cytotoxicity of carvacrol tested against SiHa (cells of human cervical cancer) and
HepG-2 (cells of human liver cancer) cells by using MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium
bromide) and LDH (lactate dehydrogenase enzyme) assays. Carvacrol compound revealed a cytotoxic effect on SiHa
and HepG-2 with dose-dependent manner in both LDH and MTT assays. The IC50 of carvacrol against HepG-2 and SiHa
cells at 48 h time point was 348.25 µm and 415.19 µm, respectively. The expression level of apoptotic regulatory gene
was examined in the presence/absence of carvacrol by using RT-PCR methods. In this study, SiHa and HepG-2 cells
treated by carvacrol showed up-regulation of p53 and Bax gene, while down regulation of Bax gene. Also, carvacrol
treated cell showed up regulation of caspase-9, -6 and -3 genes. The resultsalso showed genomic DNA fragmentations
as a hallmark of apoptosis. This study confirmed that the carvacrol induces apoptosis regulatory genes in both SiHa and
HepG-2 cells. Due to activation of apoptotic mechanism, cells undergo apoptosis andthus reduction in proliferative
potential of cells.In conclusion, this study proved the anti-proliferative effects of carvacrol against the cancer cells and
can be helpful for the development of novel anticancer drug.This study is also beneficial for herbal preparation of
anticancer drug with other possible combination of natural resources.
|
| Keyword:
|
Carvacrol, cytotoxicity, apoptosis, caspases activity,DNA fragmentation
|
| EOI:
|
-
|
| DOI:
|
-
|
| Download:
|
Request For Article
|
|
|