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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence

IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

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0975-2366
5 - Years Impact Factor

Year 2012 - 2016

Impact Factor: 1.55

Total Publications: 317

Total Citation: 491

Year 2011 - 2015

Impact Factor: 1.46

Total Publications: 326

Total Citation: 477

Year 2010 - 2014

Impact Factor: 1.3

Total Publications: 313

Total Citation: 407

Year 2009 - 2013

Impact Factor: 0.973

Total Publications: 293

Total Citation: 285

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ANTI-PROLIFERATIVE AND PRO-APOPTOTIC ACTIVITY OF CARVACROL ON HUMAN CANCER CELLS

Author: ATHEER YASEEN
Abstract: This study tested the anti-proliferative and pro-apoptotic activity of carvacrol;a compound commonly present in the essential oil of oregano and thyme.The cytotoxicity of carvacrol tested against SiHa (cells of human cervical cancer) and HepG-2 (cells of human liver cancer) cells by using MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide) and LDH (lactate dehydrogenase enzyme) assays. Carvacrol compound revealed a cytotoxic effect on SiHa and HepG-2 with dose-dependent manner in both LDH and MTT assays. The IC50 of carvacrol against HepG-2 and SiHa cells at 48 h time point was 348.25 µm and 415.19 µm, respectively. The expression level of apoptotic regulatory gene was examined in the presence/absence of carvacrol by using RT-PCR methods. In this study, SiHa and HepG-2 cells treated by carvacrol showed up-regulation of p53 and Bax gene, while down regulation of Bax gene. Also, carvacrol treated cell showed up regulation of caspase-9, -6 and -3 genes. The resultsalso showed genomic DNA fragmentations as a hallmark of apoptosis. This study confirmed that the carvacrol induces apoptosis regulatory genes in both SiHa and HepG-2 cells. Due to activation of apoptotic mechanism, cells undergo apoptosis andthus reduction in proliferative potential of cells.In conclusion, this study proved the anti-proliferative effects of carvacrol against the cancer cells and can be helpful for the development of novel anticancer drug.This study is also beneficial for herbal preparation of anticancer drug with other possible combination of natural resources.
Keyword: Carvacrol, cytotoxicity, apoptosis, caspases activity,DNA fragmentation
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