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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Antibacterial potential of ethanolic extract of broccoli(Brassica oleracea var. italica)against human pathogenic bacteria

Author: JAYAPRAKASH SOMASUNDARAM, SIVAKUMAR SIVAGURUNATHAN MONI*, HAFIZ A.MAKEEN, MOHAMMED INTAKHABALAM, SHYAM SUNDER PANCHOLI, RAHIMULLAH SIDDIQUI, MOHAMED ELTYEPELMOBARK
Abstract: Antibiotic resistance has emerged as a major public health problem to treat infectious disease. The purpose of this work is to investigate the antibacterial activity of broccoli against certain human pathogenic bacteria. In this work six human bacterial pathogens Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniawere employed for screening. The ethanol extract of the edible part of florets and stems of broccoli has shown the presence of carbohydrates, proteins, alkaloids, tannins and flavonoids. In this study the minimum inhibitory concentration (MIC) was found to be lowest for B.subtilis, 300 µg/ml among the human pathogenic bacteria that have been screened. However, in this study 500 µg/ml concentration of crude bioactive extracts (BAE) was selected as predetermined concentration for antibacterial spectrum studies. The spectrum of activity was quantified by the formation of zone of inhibition and the efficacy was compared with standard ciprofloxacin disc (5µg/disc). It was observed that the BAE showed predominant effect against B.subtilisand the least action was found against Kl.pneumoniaat 500 µg/ml concentration. The study suggesting that novel antibacterial substances can be isolated for the beneficial of human society.
Keyword: Broccoli; Antibacterial effect; Human pathogenic bacteria
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