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Evaluation of in vivo antioxidant activity of methanol extract of Buddleja asiatica
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| Author:
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RAJA. S, RAMYA. I
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| Abstract:
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Previously, we reported the in vitro antioxidant activity of methanol extract of whole plant of Buddleja asiatica. The
aim of present study was to assess the effects of oral administration of Buddleja asiatica to 18 healthy rats with
particular attention to their in vivo antioxidant property. The rats were administered with methanol extract of
Buddleja asiatica at two different doses (250mg/kg, 500mg/kg) for a period of 21 days to estimate the oxidative stress
parameters including ferric reducing ability of plasma (FRAP), lipid peroxidation, reduced glutathione (GSH) and
evaluated for antioxidant enzyme levels of catalase and superoxide dismutase in serum and tissue homogenates. FRAP
analysis showed that the high dose (500mg/kg) of methanol extract possessed better reducing power in comparison to
low dose group and control group. Pretreatment with methanol extract of Buddleja asiatica illustrated a significant
(p<0.05) increase in serum catalase and superoxide dismutase activity when compared with other groups. The extract
at the dose level of 500 mg/kg significantly restored the levels of catalase, superoxide dismutase and reduced
glutathione in selected tissue homogenates. In addition, the tested doses of methanol extract of Buddleja
asiatica (250mg/kg and 500mg/kg) possessed antioxidant activity by effectively preventing lipid peroxidation in serum and
tissues. The extent of decrease in lipid peroxidation in serum and tissue homogenates, suggested a role for the extract
in reduction of oxidative stress. The results indicated that methanol extract of Buddleja asiatica could serve as free
radical scavenger or primary antioxidants.
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| Keyword:
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Buddleja asiatica, Oxidative stress, Antioxidant enzymes, Superoxide dismutase, Lipid peroxidation
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| EOI:
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| DOI:
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