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Bioanalytical method development, validation and its application in Pharmacokinetic studies of Verapamil in the presence of Piperine in rats
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| Author:
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PRATIBHA AUTI, ASHWINI CHOUDHARY, SATISHGABHE*, KAKASAHEBMAHADIK
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| Abstract:
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During our continuingresearch of enhancing bioavailability of drugs by using piperine a known bioenhancer, a suitable
bioanalytical method was needed. Here we report the development of a simple RP-HPLC bioanalytical method for
determination of verapamil in the presence of piperine. It was carried out on RP C18 column, usingacetonitrile:
ammonium acetate buffer (60:40)(pH 5.6 adjusted with acetic acid)as the mobile phase. UV detection was at 288 nm.
The calibration plot was linear over the range studied (1-16 ug/ml) with correlation coefficient of 0.998. Limit of
detection and limit of quantitation were 0.32 ?g/mL and 0.96?g/mL, respectively. Good overall recovery (98-100%) was
obtained. The method is simple, sensitive and reproducible.The developed method was validated as per USFDA
guidelines (15) and used in the estimation of verapamil alone and in combination with piperine on oral administration in
wistar rats. It was observed that the Cmax and AUC of verapamil increased by 154% and135% respectively when it is
given in combination with piperine than verapamil alone.
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| Keyword:
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Verapamil, Piperine, Pharmacokinetics, Bioavailability, HPLC, AUC.
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| EOI:
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| DOI:
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