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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Bioanalytical method development, validation and its application in Pharmacokinetic studies of Verapamil in the presence of Piperine in rats

Author: PRATIBHA AUTI, ASHWINI CHOUDHARY, SATISHGABHE*, KAKASAHEBMAHADIK
Abstract: During our continuingresearch of enhancing bioavailability of drugs by using piperine a known bioenhancer, a suitable bioanalytical method was needed. Here we report the development of a simple RP-HPLC bioanalytical method for determination of verapamil in the presence of piperine. It was carried out on RP C18 column, usingacetonitrile: ammonium acetate buffer (60:40)(pH 5.6 adjusted with acetic acid)as the mobile phase. UV detection was at 288 nm. The calibration plot was linear over the range studied (1-16 ug/ml) with correlation coefficient of 0.998. Limit of detection and limit of quantitation were 0.32 ?g/mL and 0.96?g/mL, respectively. Good overall recovery (98-100%) was obtained. The method is simple, sensitive and reproducible.The developed method was validated as per USFDA guidelines (15) and used in the estimation of verapamil alone and in combination with piperine on oral administration in wistar rats. It was observed that the Cmax and AUC of verapamil increased by 154% and135% respectively when it is given in combination with piperine than verapamil alone.
Keyword: Verapamil, Piperine, Pharmacokinetics, Bioavailability, HPLC, AUC.
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