IJPR  artciles are Indexed in SCOPUSClick Here     Impact Factor for Five Years is 1.55 (2012 - 2016). 

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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

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0975-2366
5 - Years Impact Factor

Year 2012 - 2016

Impact Factor: 1.55

Total Publications: 317

Total Citation: 491

Year 2011 - 2015

Impact Factor: 1.46

Total Publications: 326

Total Citation: 477

Year 2010 - 2014

Impact Factor: 1.3

Total Publications: 313

Total Citation: 407

Year 2009 - 2013

Impact Factor: 0.973

Total Publications: 293

Total Citation: 285

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Stability Indicating Method Development And Validation For The Simultaneous Estimation Of Orientin, Vitexin And Luteolin In Ecbolium viride (Forssk) Alston

Author: KEERTHANA DIYYA, B. GANGARAO
Abstract: Flavonoids are a group of plant metabolites thought to provide health benefits through cell signalling pathways and antioxidant effects. The development of quality-control methods for determination of the major flavonoids in Ecbolium viride (Forssk) Alston is therefore essential for identification and evaluation of the quality of this medicinal plant. A method for simultaneous estimation of three flavonoids by reverse-phase high performance liquid chromatography (HPLC) was developed and validated. The flavonoids were analysed on a kinetex C18 100 A0 C18 column (250 mm × 4.6 mm, 5 µ particle size); using a mobile phase, consisted of binary mixture of Water pH adjusted to 3.5 with formic acid: Methanol (60:40); under the following conditions: detecting wavelength, 350 nm; flow rate, 1.0 ml/min. The HPLC system was operated at ambient temperature (30±2 0C) and the optimised method was validated. The calibration curves were linear over the range of 10- 80 µg/mL, 10-80 µg/mL and 5-35 µg/mL for orientin, vitexin and luteolin respectively (r > 0.999). The assay recoveries for three flavonoids were above 99.3%. (% RSD<1). The precision results (R.S.D.) for orientin, vitexin and luteolin were less than 1. The LOD and LOQ for orientin is 2.79 µg/mL and 9.30, for vitexin 1.97 µg/mL and 6.57 µg/mL and for luteolin 0.58 µg/mL and 1.93 µg/mL respectively. This method demonstrated good reliability and sensitivity, and can be conveniently used for the quantification of orientin, vitexin and luteolin in herb of Ecbolium viride
Keyword: Stability Indicating Methods, Orientin, Vitexin, Luteolin, Ecbolium viride
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News & Events

2017: 6.457
2016: 5.999
2015: 5.406
2014: 5.099

CiteScore 2016 -  0.09

SJR 2016 - 0.111

SNIP 2016 - 0.055

Impact Factor for five years is 1.55 (2012 - 2016)
Year 2011 - 2015 Impact Factor - 1.46 Total Publications - 326 Total Citations - 477
Impact Factor for five years is 1.3 (2010 - 2014)