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SCREENING AND IDENTIFICATION OF L- GLUTAMINASE PRODUCING BACTERIA FROM FOREST SOILS
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| Author:
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KUMBHA RAJU, M RAGHU RAM
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| Abstract:
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Abstract:
Microbial sources are the best source for the bulk production of enzymes. From that L-Glutaminase producing bacteria were isolated from forest soil. L-Glutaminase can be effectively used for treatment of patients who suffer from acute lymphoblastic leukemia and tumor cells. From three areas, forest soils were collected and screened for bacteria which produce L-Glutaminase. Fourty nine bacterial isolates were isolated based on cultural, morphological and biochemical tests. All these bacterial isolates were screened for L-glutaminase enzyme activity using rapid plate assay. Among them fourteen isolates were found to be L- glutaminase producers. Among them three isolates were finally selected based on the activity of L-glutaminase. The selected isolates were belongs to the genus Bacillus. The maximum zone was recorded by Bacillus sp. and the enzymatic activity was ranged from 7.1 U/ml - 18.9 U/ml. these isolates were used for further studies on large scale production of L-Glutaminase enzyme.
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KEYWORDS: L-glutaminase, Bacillus sps, Biochemical characterization, L-glutaminase assay.
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