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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence

IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

ISSN
0975-2366
5 - Years Impact Factor

Year 2012 - 2016

Impact Factor: 1.55

Total Publications: 317

Total Citation: 491

Year 2011 - 2015

Impact Factor: 1.46

Total Publications: 326

Total Citation: 477

Year 2010 - 2014

Impact Factor: 1.3

Total Publications: 313

Total Citation: 407

Year 2009 - 2013

Impact Factor: 0.973

Total Publications: 293

Total Citation: 285

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A review on drug: Amantadine
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ROLE OF AMIODARONE IN ACUTE MYOCARDIAL INFARACTION INDUCED VENTRICULAR TACHYCARDIA IN PATIENTS WITH LEFT ANTERIOR DESCENDING ARTERY INVOLVEMENT
Extraction optimisation, fractionation, standardisation, in vitro antioxidant and anticholinesterase activity of fractions of Indian Propolis
Development of validated stability-indicating analytical method for simultaneous estimation of Atenolol and Hydrochlorothiazide in pharmaceutical combined dosage form by High Performance Thin Layer Chromatography.
Lung Cancer in Lifelong Never Smokers-Short Review
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January - March 6 [1] 2014

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Extraction optimisation, fractionation, standardisation, in vitro antioxidant and anticholinesterase activity of fractions of Indian Propolis

Author: DR. SATHIYANARAYANAN LOHIDASAN, SADHANA NANAWARE, KAKASAHEB MAHADIK
Abstract: Study aimed at enrichment of flavonoids and phenolic contents of Indian propolis and evaluation of in vitro anticholinesterase and antioxidant activities of enriched fractions. Different enrichment techniques were used and each fraction was assessed by rapid and reproducible RP-HPLC PDA method for estimation markers such as caffeic acid phenethyl ester (CAPE), pinocembrin (PINO) and Galangin (GAL). Results showed that macerated ethanolic extract of propolis (ME) enriched with chloroform with higher content of polyphenolic (317.67 mg Gallic acid equivalents/g) and flavonoids (69.31 mg Quercetin equivalents/g) as compared to other fractions and ME (Phenolics: 20.86 mg Gallic acid equivalents/g and flavonoids: 8.39 mg Quercetin equivalents/g). Chloroform fraction was also found to have high amount of analytes and showed 2.54, 2.82 and 2.81 folds increase in content of CAPE, PINO and GAL respectively compared with ME. Chloroform fraction was found to have antioxidant activity (DPPH, IC50: 7.33 ± 0.60 µg/ml, H2O2, IC50: 22.66 ± 1.37 µg /ml, TRC, IC50: 27.86 ± 1.55 µg/ml, ICA, IC50: 52.41 ± 2.10 µg/ml) and anticholinesterase activity (IC50: 0.27 ± 0.08 mg/ml). Phenolics and total flavonoids content of propolis fractions were significantly correlated with all antioxidant activities with probability P < 0.01. The activity of the fraction may be attributed to its higher phenolic and flavonoid content. The study described an economic method of enrichment for Indian propolis that can be utilised for industrial application and the proposed fraction can be used for further evaluation in Alzheimer’s disease with its higher potential towards antioxidant and anticholinesterase activity.
Keyword: Indian propolis; Caffeic acid phenethyl ester; Pinocembrin; Galangin; Antioxidant activity; Anticholinesterase activity
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22nd National Convention of Society of Pharmacognosy & International Conference. For more details visit https://ncsp.ganpatuniversity.ac.in
Impact Factor for five years is 1.55 (2012 - 2016)
Year 2011 - 2015 Impact Factor - 1.46 Total Publications - 326 Total Citations - 477
Impact Factor for five years is 1.3 (2010 - 2014)