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Extraction optimisation, fractionation, standardisation, in vitro antioxidant and anticholinesterase activity of fractions of Indian Propolis
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| Author:
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DR. SATHIYANARAYANAN LOHIDASAN, SADHANA NANAWARE, KAKASAHEB MAHADIK
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| Abstract:
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Study aimed at enrichment of flavonoids and phenolic contents of Indian propolis and evaluation of in vitro anticholinesterase and antioxidant activities of enriched fractions. Different enrichment techniques were used and each fraction was assessed by rapid and reproducible RP-HPLC PDA method for estimation markers such as caffeic acid phenethyl ester (CAPE), pinocembrin (PINO) and Galangin (GAL). Results showed that macerated ethanolic extract of propolis (ME) enriched with chloroform with higher content of polyphenolic (317.67 mg Gallic acid equivalents/g) and flavonoids (69.31 mg Quercetin equivalents/g) as compared to other fractions and ME (Phenolics: 20.86 mg Gallic acid equivalents/g and flavonoids: 8.39 mg Quercetin equivalents/g). Chloroform fraction was also found to have high amount of analytes and showed 2.54, 2.82 and 2.81 folds increase in content of CAPE, PINO and GAL respectively compared with ME. Chloroform fraction was found to have antioxidant activity (DPPH, IC50: 7.33 ± 0.60 µg/ml, H2O2, IC50: 22.66 ± 1.37 µg /ml, TRC, IC50: 27.86 ± 1.55 µg/ml, ICA, IC50: 52.41 ± 2.10 µg/ml) and anticholinesterase activity (IC50: 0.27 ± 0.08 mg/ml). Phenolics and total flavonoids content of propolis fractions were significantly correlated with all antioxidant activities with probability P < 0.01. The activity of the fraction may be attributed to its higher phenolic and flavonoid content. The study described an economic method of enrichment for Indian propolis that can be utilised for industrial application and the proposed fraction can be used for further evaluation in Alzheimer’s disease with its higher potential towards antioxidant and anticholinesterase activity.
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| Keyword:
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Indian propolis; Caffeic acid phenethyl ester; Pinocembrin; Galangin; Antioxidant activity; Anticholinesterase activity
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