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Assessment of Antioxidant Activity of Terrestrial Actinomycetes Isolates
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| Author:
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HOTAM CHAUDHARY, ANITA GARG, BHAVANA SONI, HOTAM SINGH CHAUDHARY
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| Abstract:
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Aim- The aim of the present study was to evaluate the antioxidant activity of actinomycetes isolates. These isolates were isolated from sediment samples collected at the Bhind (geographical location: Lat. 26°36' N and Long. 78°46' E). Soil samples were taken from various places of Bhind district such as: field, near the plants root, under the well, near mining site. Actinomycetes are gram positive, aerobic bacteria with a high guanine (G) plus cytosine (C) ratio in their DNA. The actinomycetes are potential producers of antibiotics and of other therapeutically useful compounds. The bioactive secondary metabolites produced by actinomycetes include antibiotics, antitumor agents, immunosuppressive agents and enzymes. These metabolites are known to possess antibacterial, antifungal, antioxidant, neuritogenic, anti-cancer, anti-algal, anti-helmintic, anti-malarial and anti-inflammatory activities. Methods-Antioxidant compound can inhibit oxidation of vital molecules (DNA lipid and proteins) are damaged. Total 43 samples of pure culture were used in this study; which have been isolated from soil samples of Bhind region. 40 isolates obtain after primary screening out of 43 isolates. These isolates were further screened for antioxidant production by three standard methods: reducing potential assay; Fe+3 (ferric ion) reducing assay, Metal (ferrous ions) chelating assay, Hydroxyl radical (OH-) scavenging activity. Results-Five isolates AB6, AB14, AB19, AB20 and AB22 were show best result in all screening method of antioxidant activity. Conclusion- This study shows actinomycetes have properties to produced antioxidant compound against oxidative stress in cells.
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| Keyword:
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antioxidant, actinomycetes, Fe+3 (ferric ion) reducing assay, Metal (ferrous ions) chelating assay, Hydroxyl radical (OH-) scavenging activity.
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| DOI:
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