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Assessment of Biosurfactant Producing Actinomycetes Isolates from Shivpuri Region M.P. India.
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| Author:
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HOTAM CHAUDHARY, BHAVANA SONI, ANITA GARG, HOTAM SINGH CHAUDHARY
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| Abstract:
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Objective: Actinomycetes was screened and evaluated for biosurfactant production activity from shivpuri region, M.P. in India. Methods: Biosurfactant was screened by primary screening haemolytic activity, drop collapse method, oil displacement test, emulsification index and surface tension measurement. Further extraction of biosurfactants was performed with the help of chloroform: methanol (2:1) after centrifugation at10, 000 rpm for 10 min and leave it to evaporate on rotator evaporator. Final crude extracts were confirmed by oil displacement method and this crude extract of biosurfactants was lyophilized for long time preservation. Results: A total 39 actinomycetes isolates were tested and 9 isolates show positive for ? haemolytic activity which was further screened by drop collapse and oil displacement activity. Emulsification index and surface tension measurement were calculated for nine isolates. After primary screening four isolates Ash 11, Ash21, Ash 28 and Ash 39 were show positive result in total 39 isolates. Conclusion: Out of 39, Ash 39 was show highest emulsification activity (E24) 53% and reduces maximum surface tension from 139.88 to 97.55 mN/m. These screened isolates were further used for fermentation of 5-7 days for production of biosurfactant.
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| Keyword:
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Biosurfactant activity, actinomycetes, dropcollapse test, haemolytic activity, oil displacement test.
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