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FORMULATION, IN-VITRO RELEASE AND WOUND HEALING ACTIVITY OF SILVER NANOPARTICLES LOADED WITH PAPAIN
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| Author:
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DHANALAKSHMI M, SARAVANA KUMAR ARTHANARI, THENMOZHI.S, MANJULA DEVI.K
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| Abstract:
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The knowledge of the physiology of the normal wound healing trajectory through the phases of hemostasis, inflammation, granulation and maturation provides a framework for an understanding of the basic principles of wound healing. Papain is a proteolytic enzymes produced from the latex of the green papaya fruits. It has high protein hydrolyzing capacity. The papain possesses debridement, antimicrobial, antioxidant and anti-inflammatory activities. As nanoparticles possess very high surface to volume ratio the dissolution rate is increased. In The nanoparticles of silver showed high antimicrobial and bactericidal activity. The loaded papain retained high activity has improved thermal stability. In vitro release studies of the papain loaded silver nanoparticles were studied for 6 hrs for the release of papain from the silver nanoparticles. The samples were analyzed by ELISA testing. From the data obtained, it is clear that the release of papain from silver nanoparticles were found to be slow and sustained for a period of 6 hrs. The cumulative percentage release at point was 84.44% after 6 hrs. In this study, silver nanoparticles were synthesized by chemical reduction method and loaded with papain and evaluated for its wound healing activity. i.e. excision wound model were used for this purpose. In dose at 15mg of papain loaded silver nanoparticles showed significant reduction in excision wound within 8 days. The results suggested that the papain loaded silver nanoparticles possess effective wound healing activity when compared with neomycin (1%).
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| Keyword:
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Papain, Silver nanoparticles, Papain loaded silver nanoparticles, wound healing activity.
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| DOI:
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