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RP-HPLC Method Development and Validation for Simultaneous Estimation of Montelukast Sodium and Levocetirizine Dihydrochloride.
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| Author:
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UPLOADED BY-ADMIN, S. ASHOKKUMAR, M.SENTHIL RAJA, .P. PERUMAL
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| Abstract:
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In this study, reverse phase high performance liquid chromatographic method have been
developed and validated for the simultaneous determination of Montelukast and Levocetirizine
in combined pharmaceutical formulation. HPLC separation was achieved with a Phenomenex
– Luna, C18 (250 x 4.6 mm i.d., 5µ) as stationary phase and phosphate buffer (pH adjusted
to 5.5 with dil. KOH): Acetonitrile (65:35, v/v) as eluent, at a flow rate of 1.5ml/min.
UV detection was performed at 230 nm. The retention time of Montelukast and Levocetirizine
were found to 3.7 and 6.1 min respectively. Results of the analysis were validated statistically
and by recovery studies. The stability of Montelukast was maintained by carrying out
all the operations in amber coloured glass wares with minimal exposure to light. The result of
the studies showed that the proposed RP-HPLC method is simple, rapid, precise and accurate,
which can be used for the routine determination of Montelukast and Levocetirizine in bulk
and in its pharmaceutical dosage forms.
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| Keyword:
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Montelukast; Levocetirizine; HPLC; Assay.
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| EOI:
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| DOI:
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