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Antimicrobial Activity of Lawsonia Innermis L. against Common Pathogen.
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| Author:
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UPLOADED BY-ADMIN, G. B. SONARA, N. K. GHEEWALA, B. D. PATEL, M. G. SARALAYA, T. M. KERIWALA
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| Abstract:
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In vitro antimicrobial activity of Lawsonia Innermis L. leaves at varying doses of suspensions
of fresh & dried leaves were tested against the common pathogens namely Eschericia coli,
Proteus mirabilis, K. pneumonia, Pseudomonas aeroginosa and Staphylococcus aureus using
both tube turbidity standards and disc diffusion method. MIC for Escherichia coli, Klebsiellap
pneumoniae, Proteus mirabilis, Pseudomonas aeroginosa and Staphylococcus aureus
were found to be 55, 55, 75, 85 and 95 µg respectively.Antimicrobial activity of Lawsonia
Innermis L. dried leaves suspension was found to be for: Gram (-) 55-85 µg/ml and Gram (+)
: >95 µg/ml (Broth dilution method) & >95 µg/ml for both Gram (-) and Gram (+) organisms
(Disc diffusion method). Antimicrobial activity of Lawsonia Innermis L. fresh leaves suspension
by Disc diffusion method for: Eschericia coli: 10 µg/disc and Staphylococcus aureus: 25
µg/disc. So Lawsonia Innermis L. leaves have significant antimicrobial activity against the
above common pathogens. Phytochemical investigation of 70% hydroalcholic extract of Lawsonia
Innermis L. indicated that the presence of Coloring matter: Lawson (C10H6O3) as orange
crystal and Various phenolic glycosides, Coumarins, Xanthones ,Quinoids, Gallic acid, Bsitosterol,
,Flavnoids including lutiolin & its 7-O-glycosides, Isoplumbagin, tannin, Fats, Resins
had been made for antimicrobial activity.
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| Keyword:
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Lawsonia Innermis L., broth dilution, disc diffusion, Common pathogen Antimicrobial activity.
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| EOI:
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| DOI:
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