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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Antimicrobial Activity of Lawsonia Innermis L. against Common Pathogen.

Author: UPLOADED BY-ADMIN, G. B. SONARA, N. K. GHEEWALA, B. D. PATEL, M. G. SARALAYA, T. M. KERIWALA
Abstract: In vitro antimicrobial activity of Lawsonia Innermis L. leaves at varying doses of suspensions of fresh & dried leaves were tested against the common pathogens namely Eschericia coli, Proteus mirabilis, K. pneumonia, Pseudomonas aeroginosa and Staphylococcus aureus using both tube turbidity standards and disc diffusion method. MIC for Escherichia coli, Klebsiellap pneumoniae, Proteus mirabilis, Pseudomonas aeroginosa and Staphylococcus aureus were found to be 55, 55, 75, 85 and 95 µg respectively.Antimicrobial activity of Lawsonia Innermis L. dried leaves suspension was found to be for: Gram (-) 55-85 µg/ml and Gram (+) : >95 µg/ml (Broth dilution method) & >95 µg/ml for both Gram (-) and Gram (+) organisms (Disc diffusion method). Antimicrobial activity of Lawsonia Innermis L. fresh leaves suspension by Disc diffusion method for: Eschericia coli: 10 µg/disc and Staphylococcus aureus: 25 µg/disc. So Lawsonia Innermis L. leaves have significant antimicrobial activity against the above common pathogens. Phytochemical investigation of 70% hydroalcholic extract of Lawsonia Innermis L. indicated that the presence of Coloring matter: Lawson (C10H6O3) as orange crystal and Various phenolic glycosides, Coumarins, Xanthones ,Quinoids, Gallic acid, Bsitosterol, ,Flavnoids including lutiolin & its 7-O-glycosides, Isoplumbagin, tannin, Fats, Resins had been made for antimicrobial activity.
Keyword: Lawsonia Innermis L., broth dilution, disc diffusion, Common pathogen Antimicrobial activity.
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