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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Hydrophobic Interaction Chromatography- An Important Technique for separation of Proteins.

Author: UPLOADED BY-ADMIN, R. CHAUHAN, S. S. DESAI
Abstract: In this article, an overview of hydrophobic interaction chromatography (HIC) of proteins is given. After a brief introduction of protein hydrophobicity and hydrophobic interactions, we present the stationary phases that are used for this technique, salts used, comparison between reversed phase HPLC and HIC, factors affecting HIC and experimental design. Selected examples of protein purification by HIC are also presented.
Keyword: Proteins separation, hydrophobic interaction, chromatography, salting out
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0.12
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