|
Development and Validation of RP-HPLC Method for the Quantitative Estimation of Dorzolamide Hydrochloride in Ophthalmic Solution
|
|
| Author:
|
UPLOADED BY-ADMIN, C. BALA KUMAR, M. KARPAKAVALLI, B. LAKSHMI NARAYANAN, T. LINGARAJ, E. P. KUMAR
|
| Abstract:
|
Simple and sensitive method for the quantification of Dorzolamide hydrochloride in ophthalmic solution by RP-HPLC has been
developed. Isocratic separation of Dorzolamide hydrochloride was carried out using a reversed-phase column C18 (150 × 4.6
mm, 5 µm) with mobile phase consisting of methanol and buffer (pH adjusted to 3 with glacial acetic acid) in the ratio 55:45
(v/v) and quantified by UV detection at 254 nm. Analytical run time was 12 min with retention time of 3.5 min. The assay
exhibited good linear relationship accuracy and precision were over the concentration level form 50% to 150%. This method can
be used for routine analysis of Dorzolamide hydrochloride in ophthalmic solution.
|
| Keyword:
|
HPLC, validation, Dorzolamide hydrochloride, ophthalmic formulation
|
| EOI:
|
-
|
| DOI:
|
-
|
| Download:
|
Request For Article
|
|
|