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A Simple Approach to Develop and Validate RP-HPLC Method for Estimation of Quercetin in Bulk drug and Lipid Micro/Nanoparticles
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| Author:
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MUNIYAPPA GEETHA, SACHIN KANAKAGIRI, DEEPALI, SEEMA SAJJAN RATHORE, SIDDAMSETTY RAMACHANDRA SETTY
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| Abstract:
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Quercetin has anti-inflammatory, anti-oxidant, and antimicrobial properties, which necessitates its use in ulcerative colitis treatment. Despite the promising preclinical results, its utility is limited due to inefficient systemic delivery, low to moderate absorption, and limited oral bioavailability. Therefore, to achieve maximum response in terms of bioavailability, an attempt is made to prepare lipid micro/nanoparticles containing Quercetin. Hence, a simple, specific, precise, reliable, and accurate RP-HPLC method was developed and validated to quantify the drug. Chromatographic separation was achieved on Cosmicsil Amity C18 (250mm×4.6mm i.d., particle size 5mm) column with a flow rate of 1mL/min. The mobile phase consisted of methanol: buffer pH 3.0 (70:30 v/v), and the eluent was monitored at 370 nm using a UV detector. The retention time of Quercetin was found to be 5.2 min. The developed method was validated as per ICH guidelines in terms of linearity, specificity, system suitability, precision, ruggedness, robustness, and accuracy. The linearity was established over a concentration range of 2-12µg/mL with a detection and quantification limits of 0.653µg/mL and 1.981µg/mL, respectively. All the validation parameters were found to be well within the acceptance criteria. Hence, the validated method was successfully used for the estimation of Quercetin in bulk and lipid micro/nanoparticles.
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| Keyword:
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Quercetin, Lipid micro/nanoparticles, RP-HPLC, Validation, Rutin
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2023.15.04.004
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| Download:
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