|
Detection of Virulence Factor Gene Las B , PlcH and PlcN in Pseudomonas Aeruginosa Isolated Medically
|
|
| Author:
|
SHAYMAA M.MURSHED, MOHAMMED M.SALIH, SHAYMAA M.ABDUL RAZZAQ
|
| Abstract:
|
Background: Pseudomonas Aeruginosa rarely causes disease in healthy people and can cause urinary tract infections (UTIs), bacteremia, eye infections, ear infections, skin infections, central nervous system infections, and bone and joint infections.
Objectives : we aimed to study the virulence of genes : plc H, plc N and Las B on isolates of these bacteria using PCR techniques.
Materials and Methods : The study included the collection of (176) isolates from patients suffering from burns, wounds and urinary tract infections, where the samples were cultivated on different media, and the samples were diagnosed through cultural, morphological and microscopic characteristics.
Results: The prevalence of this gene las B was 78.57% in burn isolates, 85.71% in wound isolates and 85.71% in urinary tract infection isolates. As for the presence of the gene plcN that encodes for the exogenous enzyme hemolysin, , wounds and urinary tract infection isolates. As for the presence of the gene plcH that encodes for the exogenous enzyme hemolysin, the rates were 85.71% in burn isolates.
Conclusion : the detection of the gene plcN that found in 92.85% in the burn , and the another gene was plcH and the last gene was las B that destroying the elastin protein and it has a lot to do with the virulence factor of P.areuginoso in the human’s blood
|
| Keyword:
|
elastase , Las B , PlcH , PlcN , hemolysin .
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2024.16.02.002
|
| Download:
|
Request For Article
|
|
|