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Antihyperglycemic Activity Of Sida Spinosa Linn Aerial Parts In Streptozotocin-Induced Diabetic Rats
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| Author:
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PRAMOD KALDATE, VIJAYSINH SABLE, SANTOSH KIRTANE
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| Abstract:
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Objectives:The aim of the current study was to investigate theantihyperglycemic activity of various extracts of Sida spinosa Linn in STZ-induced diabetic rats.
Methods:The aerial parts of Sida spinosa (family: Malvacea) were extracted by successive solvent extraction using petroleum ether, ethyl acetate, ethanol and water. The extracts were tested for presence of phytochemical constituents and were assessed for acute oral toxicity in rats.The oral glucose tolerance test was performed in rats using various extracts of Sida spinosa. Experimental diabetes was induced in rats by intraperitoneal administration of streptozotocin (STZ) (45 mg/kg) in Wistar rats. Animals showing blood glucose level more than 200 mg/dl were considered diabetic. Diabetic rats were then treated with petroleum ether, ethyl acetate, ethanol and aqueous extracts of Sida spinosa orally at the dose of 200 mg/kg body weight.Glibenclamide was used as a reference drug at a dose of 10 mg/kg b.w.The animals were treated with extracts& standard drug for 21 days. At the end of experiment blood was withdrawn to measure glycosylated haemoglobin (HbA1c), blood glucose, total protein and lipid profile. The change in body weight and kidney weight were also measured. The urine output is measured throughout the study.
Results: Phytochemical screening revealed the presence of steroids, glycosides, flavonoids and carbohydrates in the various extracts of Sida spinosa. Acute oral toxicity study showed that the extracts are non-toxic at the dose of 2000 mg/kg. All the extracts showed decrease in blood glucose level in diabetic rats. Among four extract ethanolic extract showed a significant (p < 0.001) reduction in blood glucose. The extract showed a significant reduction in the blood glucose, lipid profile, HbA1c and significant increase in serum total protein at a dose of 200 mg/kg when compared with diabetic rats at the end of 21 days.Sida spinosa extract treatment showed marked decrease in diabetes induced polyuria, hyperglycaemia, hyperlipidemia and renal hypertrophy.
Conclusion:The findings of the present study indicate that Sida spinosa extracts possess significant antihyperglycemic and antihyperlipidemic potential which may prove beneficial in the treatment of diabetes and associated complications.
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| Keyword:
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Diabetes, Sida spinosa, streptozotocin, glibenclamide
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.722
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| Download:
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