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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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HPTLC-MS Based Method Development For Quantification Of Curcumin In Different Turmeric Samples

Author: , YASHWANT D. NAKHATE, KANCHAN S. TALEKAR, M. P. PURANIK, PRANALI S. KALAMBE
Abstract: The present study reported a simple, sensitive, and fast HPTLC method development and quantification of curcumin in C.longa L. The separation was performed on TLC aluminium plates precoated with silica gel 60 F254. Good separation was achieved in the mobile phase of toluene : acetic acid (8 : 2, v/v) at Rf = 0.52 for curcumin and the compound was quantified at its absorbance maxima (?max ) at 419 nm. The objective of the study to perform the HPTLC method development and validation for quantification of curcumin in various marketed turmeric samples. Method validation is done as per ICH guidelines. The linear regression analysis for calibration plots produced R2 = 0.999 for Corcumin. Recording of characteristic molecular ion peaks of compound A1 and A2 at 544.5 and 308.3 by mass spectroscopy meets the molecular weight of actual compound.
Keyword: Curcuma longa L, Turmeric, Curcumin , HPTLC, LC-MS, Quantification
DOI: https://doi.org/10.31838/ijpr/2023.15.02.006
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