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Action of Ultra-low Dose Medicine on Oxidative Stress and Cell Stiffness of Microglial Cells In Vitro with Actin Filaments Reorganization
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| Author:
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GAËL RUNEL, ANNE PAUMIER, JUSTINE VERRE, ANNA CATTE, SANDRA TRIBOLO, JULIEN CHLASTA, NAOUAL BOUJEDAINI
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| Abstract:
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Microglia are the resident macrophage population of the central nervous system. So, microglial cells are a powerful model for studying the mechanism of inflammatory responses. For 70 years, Oscillococcinum®, a homeopathic medicine, has been used for influenza-like illnesses. However, to date its mechanism of action is still unknown. In this study, we investigated the effect of Oscillococcinum 200K dilution in lipopolysaccharide-activated microglial cells in vitro. Inflammation with lipopolysaccharide induces a high production of reactive oxygen species in the cells and Oscillococcinum 200K dilution significantly reduced this production. In order to explain how Oscillococcinum 200K dilution induced this anti-oxidant effect, we decided to study its effect on cell stiffness using Atomic Force Microscopy. In the literature, Atomic Force Microscopy data on cells provides evidence of mechanical changes of cellular structures with different treatments. Atomic Force Microscopy data showed that Oscillococcinum 200K dilution immediately increased the cell stiffness of microglial cells and enhanced the reorganization of actin filaments.
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| Keyword:
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Ultra-low dose, Oscillococcinum, AFM, stiffness, microglial cells, anti-oxidant
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2022.14.01.016
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| Download:
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