|
Analytical Method Development And Validation Of Perindopril In Bio Relevant Dissolution Medium Using Rp-Hplc
|
|
| Author:
|
MERUGU MADHUSUDHAN, DR.RAKESH KUMAR JAT, DR.GAMPA VIJAY KUMAR
|
| Abstract:
|
A new method was established for estimation of Perindopril by RP-HPLC method by using Biorelevent Dissolution Media (FaSSIF). The chromatographic conditions were successfully developed for the separation of Perindopril by using YMC C18 column (4.6×150mm) 5µ, flow rate was 0.6 ml/min, mobile phase ratio was Water: meoH (20:80% v/v), detection wavelength was 320nm. The instrument used was WATERS HPLC Auto Sampler, Separation module 2695, photo diode array detector 996, Empower-software version-2. The retention times were found to be 2.497 mins. The % purity of Perindopril was found to be 99.94%.The system suitability parameters for Perindopril such as theoretical plates and tailing factor were found to be 4187, 1.5. The analytical method was validated according to ICH guidelines (ICH, Q2 (R1)). The linearity study of Perindopril was found in concentration range of 20µg-100µg and correlation coefficient (r2) was found to be 0.999, % recovery was found to be 99.95%, %RSD for repeatability was 0.2, % RSD for intermediate precision was 0.1. The precision study was precision, robustness and repeatabilty.LOD value was3.06 and LOQ value was 10.14.
|
| Keyword:
|
YMC C18, Perindopril, RP-HPLC.
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2020.12.04.713
|
| Download:
|
Request For Article
|
|
|