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Hplc Method Development And Validation For The Estimation Of Deflazacort In Nanosponges Formulations
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| Author:
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SARVESH MISHRA, NEERAJ SHARMA AND SHAILESH JAIN
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| Abstract:
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A simple, rapid and selective RP-HPLC method was developed for the estimation of Deflazacort in bulk drug and (In-house) nanosponges formulation. Deflazacort is a corticosteroid which is an oxazoline derivative of prednisolone, characterized by a high binding affinity to tissue glucocorticoid receptors and exert antiinflammatory and immunosuppressive effects. Deflazacort significantly inhibits both the proliferation of mononuclear cells derived from human peripheral blood, and the release of inflammatory cytokines by these cells. The separation was achieved on Thermo C18 analytical column (250 mm × 4.6 mm i.d., 5.0 µm) using 20mM KH2PO4: Acetonitrile in the ratio of 20:80v/v (pH adjust with 4.0 with OPA) as mobile phase and at a flow rate of 1.0 ml/min. Detection was carried out using a UV detector at 242nm. The total chromatographic analysis time per sample was about 15min with DFZC eluting at retention time of about 4.085 ± 0.002min. The method was validated for accuracy, precision, specificity, linearity and sensitivity. Validation studies demonstrated that this HPLC method is simple, specific, rapid, reliable and reproducible. The developed and validated method was successfully applied for the quantitative analysis of DFZC in pharmaceutical formulation. The high recovery and low relative standard deviation confirm the suitability of the proposed method for the determination of DFZC (In-house) nanosponges formulation.
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| Keyword:
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RP-HPLC, Deflazacort, Method development, Method validation
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.710
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| Download:
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