|
Effect of BAP on in vitro propagation of Taxus contorta – an endangered multipurpose tree of the Western Himalayas
|
|
| Author:
|
SUSHANT SHARMA, YASHASWI SINGH, MAHIMA, AMBIKA, SEEMA SINGH, AJAY THAKUR
|
| Abstract:
|
Taxus contorta is an invaluable tree species for its anti-cancerous properties which is experiencing severe deterioration in population across its range in India. Categorized as Endangered in the IUCN Red List of Threatened Species and listed under CITES Appendix II. Harvesting for medicinal use coupled with slow growth and low natural regeneration are some of the reasons for its dwindling natural population. The leaves and bark of T. contorta are being utilised for extraction of Paclitaxel, which is anti-neoplastic, inhibits growth of cancerous cells, and is used in the treatment of breast and ovarian carcinomas. Biotechnological tools such as tissue culture have been used for rapid clonal propagation, multiplication and conservation of such threatened and vulnerable species. The current study focusses on the establishment of aseptic cultures of T. contorta in vitro and the effect of BAP on bud induction and shoot proliferation. Treatment with 0.1% HgCl2 was found to be optimum for aseptic culture establishment. ½ MS medium supplemented with 8.88 µM and 2.22 µM BAP were found to be optimum for in vitro bud induction and shoot proliferation, respectively.
|
| Keyword:
|
Taxus contorta, Endangered, biodiversity conservation, in vitro propagation, Taxol
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2020.12.04.701
|
| Download:
|
Request For Article
|
|
|