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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

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IJPR included in UGC-Approved List of Journals - Ref. No. is SL. No. 4812 & J. No. 63703

Published by : Advanced Scientific Research
ISSN
0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Marker Based Standardization of Tinospora cordifolia Stem Extract by HPTLC

Author: UPLOADED BY-ADMIN, V. SIVAKUMAR, M. S. DHANA RAJAN
Abstract: A simple and reproducible high performance thin layer chromatography method for the determination of berberine in methanol extract of Tinospora cordifolia was developed and is described. This method involves separation of compounds by TLC on pre-coated silica gel 60F 254 plates with a solvent system of butanol: ethyl acetate: acetic acid: water (3:5:1:1) and scanned using densitometric scanner in UV reflectance photo mode at 366nm. The berberine content of 0.23% w/w was observed in methanol extract of Tinospora cordifolia test sample. The proposed method being precise and sensitive can be used for detection, monitoring and quantification of berberine in Tinospora cordifolia.
Keyword: HPTLC, Tinospora cordifolia, Berberine.
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