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Bioanalytical Method Development And Validation Of Cabozantinib In Rat Plasma By Using Lc-Ms/Ms
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| Author:
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SHAILAJA.KONA, GANDLA.KUMARASWAMY
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| Abstract:
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Background; Anticancer drug use has increased significantly over the last few decades, and until recently, only one method utilizing human plasma had been developed. There is currently no method for quantifying Cabozantinib in rat plasma using LC-MS/MS. In the present work a New LC-MS/MS method for the Quantification of Cabozantinib in Rat plasma with less processing time, sensitive and less runtime was developed.
Results. The method was validated over aconcentration of eight working standards solutions ranging from 5.00 to 1000 ng mL-1 with correlation coefficient (r2 0.9896). The Run time is about 3.0 minutes. The average extraction recoveries were 100.04 for Cabozantinib and 102.8 for Naproxen for three different quality control concentrations. The coefficient of variation for intra- and inter-batch testing was =15%.
Conclusion A sensitive, selective and robust Liquid Chromatography- Mass Spectrometry method for the determination of Cabozantinib in Rat Plasma has been developed and validated using Naproxen as an internal standard.
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| Keyword:
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Cabozantinib, Acetonitrile, lower limit of quantification, Rat plasma, internal standard
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.689
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| Download:
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