|
Phytochemical and Cytotoxic effects of silver nanoparticles synthesized from Chromolaena odorata plant on MG-63 Cell line.
|
|
| Author:
|
, S.JAGATHEESH, D.ANANDHI, K.REVATHI
|
| Abstract:
|
Nanoparticles have been in demand because of their use in varied fields like catalysis, electronics, energy, chemistry and medicine. With the use of wet chemical methods metallic nanoparticles are traditionally synthesized, where the chemicals castoff may be flammable and toxic. Here we present an environment friendly synthesis of silver nanoparticles with the leaf extract of Chromolaena odorata. In the present study phytochemical screening has been carried out for phyto components like carbohydrates, terpenoids, resins, saponins, tannins and alkaloids in the Chromolaena odorata extract by standard protocols and antibacterial activities of Chromolena odorata leaf extract of silver nanoparticles prepared from extracts. Qualitatively analysed the phytochemical constituents of C.odorata and confirmed the presence of various phytochemicals in the Ethanol extract. The In vitro screening of AgNps of C.odorata, showed cytotoxicity against all the cell lines within the concentration ranging of 5 µg/ml to 100 µg/ml. All the concentrations of AgNps showed cytotoxicity ranging from 25 – 90 % depending on the concentration of AgNps. The characteristic feature of apoptosis is DNA fragmentation. Therefore the DNA fragmentation assay confirmed induced apoptosis by C.odorata AgNps. Increased DNA fragmentation was apparent in MG-63 cells at the 25 and 50 µg/ml.
|
| Keyword:
|
Chromolaena odorata, phytochemical screening, silver nanoparticles; anticancer activity. MG-63Cell line.
|
| EOI:
|
-
|
| DOI:
|
https://doi.org/10.31838/ijpr/2021.13.03.246
|
| Download:
|
Request For Article
|
|
|