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Method Development And Validation For Metal Analysis Of Herbal Drug (Saraswatarishta) Using Inductive Coupled Plasma Optical Emission Spectroscopy
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| Author:
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SRIDHAR BABU GUMMADI, SNIGDHA DAMIREDDY
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| Abstract:
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A precise, accurate and reproducible method was developed and validated by using Inductive Coupled Plasma Optical Emission Spectroscopy (ICP-OES) for the estimation of heavy metals in Saraswatarishta. Linearity was established by plotting a calibration curve between emission intensity vs corresponding metal concentrations. Correlation coefficient was calculated from the linear regression analysis proved that the developed method obeys linearity. Specificity was established based upon detection of metal concentrations in blank solution, sample and spiked sample solution. The sample solution and blank were reported to be completely devoid of any metal content proving method specificity. The system precision and method precision was evaluated each for 6 repetitive times. The %RSD values for the generated data were below the accepted criteria. Hence the developed method was precise. Accuracy of the proposed method was based upon determination of spiked sample recoveries. The obtained mean spike recoveries are shown in tables and were within the ICH acceptance criteria of 80% to 120%. Hence the developed method was accurate. Ruggedness of the proposed method was determined and the obtained spike recoveries were within the ICH acceptance criteria of 95% to 110%. Hence the developed method was rugged and the results are shown in tables. The heavy metals in the given sample were below detection level in the given sample.
Correlation coefficient was calculated from the linear regression analysis proved that the developed method obeys linearity for Saraswatarishta.
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| Keyword:
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Saraswatarishta, Inductive coupled plasma optical emission spectroscopy,Method development, Validation
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| EOI:
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-
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.684
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| Download:
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