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Design, Formulation and Evaluation of Cabozantinib Loaded Liposome by RP-HPLC
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| Author:
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K.B.BHANSALI, S.N.NAGOBA, S.R.HYAM
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| Abstract:
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Liposomes were created to deliver drugs to specific locations. The development of an analytical approach to determine the medication contained in the liposome is critical. The goal of the research was to create a reverse phase high performance liquid chromatography (RP- HPLC) method for determining cabozantinib concentration. The method was developed using an Agilent zorbax eclipse XDB C18 column (4.6 x 250 mm, 5m) with a mobile phase of methanol:0.1 percent OPA (Ortho phosphoric acid) in an 80:20 percent v/v ratio. The mobile phase flow rate was kept constant at 0.7 ml/min. The detection was done at a wavelength of 244 nm. The approach is quick and cost-effective due to the low flow rate and short retention time. The linearity, accuracy, precision, robustness, LOD, and LOQ properties of this approach were all validated. At 80, 100, and 120 percent, the percent recovery was found to be 102.54, 100.65, and 101.04. The percent RSD of intra-day precision was determined to be 0.04, 0.04, and 0.03 whereas the percent RSD of interday precision was 0.07, 0.12, and 0.08. The method that was devised was unique. Cabozantinib had a retention time of 3.651 minutes. 0.0609ppm and 0.1845ppm were found to represent the LOD and LOQ, respectively.
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| Keyword:
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Method development, validation, liposomes, Cabozantinib, RP- HPLC, ICH.
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2020.12.04.675
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| Download:
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