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Characterization of Nanoparticle-encapsulation of ß-carotene Isolated from Daucus carota
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| Author:
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, MUHAMAD TASWIN, SONLIMAR MANGUNSONG, AZZA HANIF HARISNA, DWI WAHYU NUGROHO, PUTRI HAWA SYAIFIE, ETIK MARDLIYATI, SATHEESH BABU NATARAJAN
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| Abstract:
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ß-carotene is a potent lipophilic nutraceutical which has low bioaccessibility and bioavailability caused by poor water solubility, stability, sensitivity to light, temperature and pH. Nano-encapsulation can protect it from degradation and retain a compound's functional property . The aim of this study is to characterize the ß-carotene from Daucus carota root which is nano-encapsulated with the chitosan matrix with TPP. ß-carotene was isolated from Daucus carota root origin from local vegetable plantations, Palembang, Indonesia. Then, it was encapsulated by entrapping it with a chitosan-TPP solution using inclusion complexation method with variants 0.1%, 0.25%, and 0.5%, followed by ß-carotene. Size and morphology of ß-carotene were analyzed using particle size analyser (PSA) and transmission electron microscopy (TEM). Results showed that the particle size of the encapsulation of the chitosan increases as long as the ß-carotene concentration. Only the variant 0.5% detects the level of ß-carotene as much as 0.03 mg/kg. ß-carotene encapsulated with chitosan-TPP appear in a spherical shape with a size of 20 nm.
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| Keyword:
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Chitosan, Nanoparticles ,Daucus carota, PSA, TEM, and ß-carotene
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.03.173
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| Download:
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