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The Study and Determination of Maximum Capacity of Pichia pastori Yeast Metabolic Network in Producing Penicillin
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| Author:
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SEYED BEHZAD ROUHIPOUR, HANIYE JAFARI GILDEH
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| Abstract:
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Penicillin, also known as the acronym PEN, is a category of antibiotics produced by penicillium and aspergillus species. Penicillin is one of the first drugs produced to treat the diseases caused by pathogenic bacteria that are still applied comprehensively. Nowadays, attempts are being made to produce penicillin through recombinant DNA technology and the use of heterologous hosts on an industrial and large scale that consequently, the microorganisms producing penicillin will not merely be limited to penicillium and aspergillus species. One interesting method is producing penicillin by the use of a heterologous host. The purpose of this research was to study the metabolic capacity of Pichia pastoris, a type of methylotrophic yeast, to produce penicillin using its genome scale model. The model applied is based on the modeling on the mentioned term and has 670 genes and 1385 reactions; this study has proceeded the capacity of the metabolic network through adding the reactions of the process from penicillin production to the metabolic network of Pichia pastoris, and FBA, FVA,…, Robustness analysis have been used in this path. Also, the network capacity has been studied using the mentioned analysis in various conditions of medium and different carbon sources.
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| Keyword:
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Systemic Biology, Penicillin, Pichia pastoris, Scale Genome Model, Heterologous Host
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| EOI:
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| DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.778
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| Download:
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