Evaluation of Humoral Immune Response in Bovine Mastitis Causing Staphylococcus aureus Biofilm Vaccine
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Author:
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SHOAIB AHMAD SHAKHES, MOHAMMAD JAVID FAZLY, ABDUL QADER POOYAN, NAZIR AHMAD TOOKHY, D.RATHNAMMA, SHRIKRISHNA ISLOOR
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Abstract:
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Bovine mastitis is one of most costly and complex diseases of the dairy industry worldwide. The present study was designed to evaluate the bovine mastitis causing S. aureus BF vaccine to study the humoral immune response in cattle vaccinated with S. aureus BF vaccine in comparison with FC vaccine by ELISA. In all, eighteen cattle in early lactation which were free from mastitis were subjected to trials. Bentonite clay based S. aureus BF and FC vaccines were administered at 0, 30 and 60 days using FIA (Freund’s Incomplete Adjuvant) in the first shot. In indirect ELISA, serum IgG level was significantly higher in BF vaccinated than FC vaccinated groups. The increased levels of IgG in the BF vaccinated sera samples were due to humoral immune responses indicated the superiority of S. aureus BF vaccine over FC vaccine.
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Keyword:
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Mastitis; Bovine; Staphylococcus aureus; Biofilm vaccine; Humoral Immune Response
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EOI:
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-
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DOI:
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https://doi.org/10.31838/ijpr/2021.13.01.773
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