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INTERNATIONAL JOURNAL OF PHARMACEUTICAL RESEARCH

A Step Towards Excellence
Published by : Advanced Scientific Research
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0975-2366
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IJPR 9[3] July - September 2017 Special Issue

July - September 9[3] 2017

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Pharmacognostical, Phytochemical and HPTLC Study of Indian Medicinal Plant Ludwigia octovalvis

Author: STUTI PANDEY, ALAKH N.SAHU, , MANMATH K.NANDI
Abstract: Medicinal plants are treasure of Ayurveda and recently researchers are emphasizing on medicinal plants to obtained new phyto-pharmaceuticals to counter the various diseases. The Indian system of medicines, recommend extracts of medicinal plants instead of natural phytomolecules for the treatment of various anomalies and diseases, by considering that the cumulative effect of extract is more effective over the individual phytomolecules. The medicinal plant Ludwigia octovalvis, commonly known as Mexican primerse – willow (Onagraceae) is used traditionally for the treatment of various diseases like edema, nephritis, hypotension, anti-malarial, dysentery, diarrhea, diabetes, headache, fever, toxemia, as carminative, laxative, vermifuge, astringent, anthelmintic, in nervous disorders, as analgesic, in rheumatic pain and for swollen glands. Collection of plant material was bringing out and its morphological, physicochemical and phytochemical studies (preliminary qualitative and quantitative analysis) and advanced chromatographic study like HPTLC profiles of the hydro-alcoholic extract were performed. Also various pharmacognostical standards for Ludwigia octovalvis have been established in the present investigation. The hydro- alcoholic extract of Ludwigia octovalvis studied for phytochemicals and confirmed the presence of phytochemical like carbohydrate, alkaloid, saponin, flavonoids and phenolics. The quantitative estimation of antioxidant bio-components like total phenolics and total flavonoid contents were calculated by UV- Visible spectroscopy method and determined as 5.15 mg/g gallic acid equivalent and 43.9 mg/g quercetin equivalent respectively. Further, the hydro-alcoholic extract of Ludwigia octovalvis was standardized by HPTLC method by using various marker compounds and confirmed the presence of bioactive constituents as lupeol, quercetin, gallic acid and stigmasterol. These studies may be used as reference for the development of authentic monograph for this drug and also helpful for recognition and standardization of whole plant of Ludwigia octovalvis from the other species of Ludwigia.
Keyword: Ludwigia octovalvis, quercetin, gallic acid, TLC & HPTLC.
DOI: https://doi.org/10.31838/ijpr/2021.13.01.759
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